- Mar 30 Antonio de Cabezón, Spanish blind organist and composer, born in Castrillo Mota de Judíos, Spain (d. 1566)
- Jun 9 Nicolaas van Nieuwland, Dutch clergyman (1st Catholic Bishop of Haarlem. 1561-69), born in Maartensdijk, Bishopric of Utrecht, Holy Roman Empire (now Netherlands) (d. 1580)
- Jul 22 Alessandro de' Medici, Duke of Florence (d. 1537)
- Oct 6 John Caius, English physician, born in Norwich, England (d. 1573)
- Oct 6 Rowland Taylor, English clergyman, born in Northumberland, England (d. 1555)
- Oct 25 Renée of France, French princess and Duchess of Ferrara, born in Château de Blois, Touraine, France (d. 1574)
- Oct 28 Francis Borgia, 4th Duke of Gandía and Jesuit priest, born in Duchy of Gandía, Kingdom of Valencia, Spain (d. 1572)